Project since 2025

Sweet Proteins

Nature-inspired sweetness through precision fermentation — intense taste, protein metabolism, and a path beyond sugar and chemical sweeteners.

Sweet protein molecular concept visualization

Why sweet proteins matter

Sweet proteins such as thaumatin can be thousands of times sweeter than sucrose. They deliver strong sweetness while being metabolized as proteins rather than as sugars or synthetic sweetener molecules.

That makes them attractive for reduced-sugar foods and beverages — without the same concerns often associated with sugar or many chemical alternatives, such as calorie load, dental health, or metabolic side effects.

The challenge

Today, many sweet proteins still depend on plant extraction. Supply is limited, seasonal, and hard to expand to the scale of a global ingredients market.

Our approach

WFFI develops sweet proteins through precision fermentation, using microbial hosts to produce nature-inspired sweetness in a more controllable and scalable way.

The key bottleneck

Thermostability remains critical. Proteins must keep their taste performance through pasteurization, baking, formulation, and storage.

Research focus

Our work aims to make fermentation-derived sweet proteins practical for real food systems — not only expressive in the lab, but stable under industrial processing conditions.

  • Strain engineering — improve expression efficiency in yeast hosts
  • Thermostability design — strengthen protein performance under heat and processing stress
  • Application readiness — connect molecular design with food use cases and scale-up pathways
01

Host Engineering

Build yeast strains for sweet protein production

02

Protein Design

Optimize structure for stability and sweetness

03

Heat Stress Testing

Evaluate performance under processing conditions

04

Functional Screening

Compare sweetness retention after treatment

05

Pilot Scale-up

Advance promising candidates with NewPro

06

Food Applications

Explore formulation routes for real products

3D molecular structure of a sweet protein

From molecular design to use

By combining protein engineering with fermentation process development, we aim to create sweet proteins that remain functional through heat treatment and industrial handling.

Research direction and AI-assisted protein redesign are driven at WFFI in Wageningen. Pilot production and experimental validation can be advanced with NewPro, helping move concepts toward practical manufacturing.

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